澳系进口荷斯坦牛IL8基因遗传多态性与体细胞评分及泌乳性状的关联分析

doi:10.11843/j.issn.0366-6964.2013.05.004

畜牧兽医学报 ›› 2013, Vol. 44 ›› Issue (5): 690-696.doi: 10.11843/j.issn.0366-6964.2013.05.004

澳系进口荷斯坦牛IL8基因遗传多态性与体细胞评分及泌乳性状的关联分析

张美荣¹，陈丹¹，廖想想¹，毛永江^1，2*，刘坤³，陈亮³，王杏龙¹，杨章平¹，杨利国²

（1. 扬州大学动物科学与技术学院，扬州 225009； 2. 华中农业大学动物遗传育种与繁殖教育部重点实验室，武汉 430070； 3. 上海牛奶集团海丰奶牛场有限公司，大丰 224100）

收稿日期:2012-10-12 出版日期:2013-05-23 发布日期:2013-05-23
通讯作者: 毛永江，男，博士，副教授，E-mail: maoyongjiang@gmail.com
作者简介:张美荣（1988- ），女，江苏如皋人，硕士生，主要从事动物遗传资源评价、保护与利用研究，E-mail: meirongzhang2010@126.com
基金资助:
江苏省苏北专项（BN2011016）；江苏高校优势学科建设工程资助项目（PAPD）；中国博士后基金（20110491169）；扬州大学科技创新培育基金（2011CXJ059）

Genetic Polymorphism of IL8 Gene and Its Association with Milk Traits and SCS in Holstein Imported from Australia

ZHANG Mei-rong¹, CHEN Dan¹, LIAO Xiang-xiang¹, MAO Yong-jiang^1,2*, LIU Kun³, CHEN Liang³, WANG Xing-long¹, YANG Zhang-ping¹, YANG Li-guo²

（1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China；2. Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, China; 3.Haifeng Dairy Cattle Farm Co. LTD, Shanghai Dairy Group, Dafeng 224100, China）

Received:2012-10-12 Online:2013-05-23 Published:2013-05-23

摘要/Abstract

摘要：

以海丰奶牛场来自72个公牛家系588头胎澳系进口荷斯坦牛为试验材料，分析白介素8（Interleukin-8，IL8）基因2个突变位点对日产奶量、乳脂率、乳蛋白率、305 d产奶量、305 d乳脂量、305 d乳蛋白量及体细胞评分（SCS）7个性状的影响，寻找可用于生产应用的分子标记。用PCR-SSCP技术对IL8基因的遗传多态性进行分析，采用最小二乘模型分析多态位点与各泌乳性状及SCS的相关性。结果表明，IL8基因5′侧翼区序列-180位点发生了G→A的突变，检测到GG、GA和AA 3种基因型，频率分别为0.226、0.476和0.298，等位基因G和A的频率分别为0.464和0.536；该位点突变对305 d脂肪产量有显著影响，而对其它泌乳性状及SCS均无显著影响。GG基因型305 d脂肪产量显著高于GA基因型(P<0.01)，而AA基因型305 d脂肪产量与GG、GA基因型之间无显著差异(P>0.05)。IL8基因2 789发生A→G的突变，共检测到GG、GA和AA 3种基因型，频率分别为0.297、0.506和0.197，等位基因G和A的频率分别为0.550和0.450；该突变位点对测定日乳脂率、乳蛋白率、305 d校正奶量和SCS有显著影响(P<0.05)。AA基因型乳脂率极显著高于GA、GG基因型(P<0.01)；AA基因型乳蛋白率极显著高于GA基因型(P<0.01)；GG基因型的体细胞评分(SCS)极显著高于GA、AA基因型(P<0.01)；对于305 d校正奶量而言，GA基因型显著高于AA基因型(P<0.05)。IL8基因遗传突变对澳系进口荷斯坦牛泌乳性状和乳房炎抗性有较大的遗传效应，可用于其分子标记辅助选择。

Abstract:

The effect of two mutations in Interleukin-8 (IL-8) gene for 588 Holstein cows from 72 bull families of Haifeng dairy cattle farm imported from Australia were investigated on 7 milking performances: tested day milk yield, milk fat percentage, milk protein percentage, 305 d corrected milk yield, 305 d milk fat yield, 305 d milk protein yield and somatic cell score (SCS). The aim was to explore molecular marker in the breeding of dairy cattle. The polymorphism of IL8 gene was detected by polymerase Chain reaction-single strand conformation polymorphism (PCR-SSCP) technique. The association between polymorphic sites of IL8 gene and milking performances , SCS was analyzed by the least square mean model. The results showed that a mutation from G to A was identified in the 5′ upstream of -180(G/A), the genotypic frequencies of GG, GA and AA were 0.226, 0.476 and 0.298, respectively. The gene frequencies of G and A were 0.464 and 0.536, respectively. No significant effects of mutation were observed on milking performances and SCS except on 305 d milk fat yield. The 305 d milk fat yield for GG genotype was higher than GA (P<0.05). A mutation from A to G was identified in 2 789 of IL8, and the genotypic frequencies of GG, GA and AA were 0.297, 0.506 and 0.197, respectively. The gene frequencies of G and A were 0.550 and 0.450. This mutation showed significant effects on tested day milk fat percentage, milk protein percentage, 305 d corrected milk yield and SCS. The AA genotype had greatly significant higher milk fat percentage than GG and GA genotypes (P<0.01). Milk protein percentage for AA was greatly significant higher than that GA (P<0.01). GG genotype had greatly significant higher SCS than GA and AA (P<0.01). 305 d corrected milk yield for GA was significant higher than AA (P<0.05). The polymorphisms of IL8 gene had a greatly genetic effects on milking traits and mastitis resistance, so this gene could be a useful genetic marker for Holstein cows imported from Australia.

中图分类号:

S823.9⁺1.2

张美荣，陈丹，廖想想，毛永江，刘坤，陈亮，王杏龙，杨章平，杨利国. 澳系进口荷斯坦牛IL8基因遗传多态性与体细胞评分及泌乳性状的关联分析[J]. 畜牧兽医学报, 2013, 44(5): 690-696.

ZHANG Mei-rong, CHEN Dan, LIAO Xiang-xiang, MAO Yong-jiang, LIU Kun, CHEN Liang, WANG Xing-long, YANG Zhang-ping, YANG Li-guo. Genetic Polymorphism of IL8 Gene and Its Association with Milk Traits and SCS in Holstein Imported from Australia[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 690-696.

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参考文献

［1］SEEGERS H, FOURICHON C, BEAUDEAU F. Production effects related to mastitis andmastitis economics in dairy cattle herds［J］. Vet Res, 2003,34:475-491.

［2］RUEGG P L. Investigation of mastitis problems on farms［J］. Vet Clin Food Anim Pract, 2003,19(1):47-73.

［3］DINGWELL R T, GELTON D F, LESLIE G E. Management of the dry cow in control of peripartumdisease and mastitis［J］. Vet Clin Food Anim Pract, 2003,19(1):235-265.

［4］张亚琴，杨章平，毛永江，等.运用多座位外显方差法分析中国荷斯坦牛CXCR1和IL8基因与乳房炎易感性的关系［J］.畜牧兽医学报，2011，42(12): 1696-1703.

［5］SCHUGGEN Y H, WILSON D J, WELCOME F, et al. Monitoring udder health and milk quality using somatic cell counts［J］. Vet Res, 2003,34:579-596.

［6］郭秀兰,王康宁.乳中体细胞数在牛奶生产中的监控作用［J］.中国饲料,2003,20:33-34.

［7］冀德君,陈仁金,杨章平,等.中国荷斯坦奶牛IL-8基因SNP-180A/G显著影响体细胞评分［J］.中国农业科学,2012,45(7):1378-1391.

［8］RUPP R, BOICHARD D. Genetic parameters for clinical mastitis, somatic cell score, production, udder type and milking easy in first lactation Holsteins［J］. J Dairy Sci, 1999, 82: 2198-2204.

［9］LARSEN C G, ANDERSON A O, APPELLA E, et al. The neutrophil activating protein (NAP-i) isalso chemotactic for T lymphocytes［J］. Science, 1989, 243(4897): 1464-1466.

［10］HUBER A R, GUNGEL S L, TODD R T, et al. Regulation of transendothelial neutrophil migration by endogenous interleukin-8［J］. Science, 1991, 254(5028): 99-102.

［11］GOCH A E, POLVERINI P J, GUNGEL L, et al. Interleukin-8 as a macrophage-derived mediator of angiogenesis［J］. Science, 1992, 258(5089): 1798-1801.

［12］HARADA A, SEGIDO N, AGAHOSHI T, et al. Essential involvement of interleukin-8 (IL-8) in acute inflammation［J］. J Leugocyt Biol, 1994, 56(5): 559564.

［13］LEE J W, BANNERMAN D D, PAAPE M J, et al. Characterization of cytokine expression in milk somatic cells during intramammary infections with Escherichia coli or Staphylococcus aureus by real-time PCR［J］. Vet Res, 2006, 37(2):219-229.

［14］WATANABE A, YAGI Y, SHIONO H, et al. Effects of intramammary infusions of interleukin-8 on milk protein composition and induction of acute-phase protein in cows during mammary involution［J］. Can J Vet Res, 2008, 72(3):291-296.

［15］BANNERMAN D D, GAUF A C W, PAAPE M J, et al. Comparison of Holstein and Jersey innate immune responses to Escherichia coli intramammary infection［J］. J Dairy Sci, 2008, 91(6):2225-2235.

［16］GUNTHER J, SHUZHEN L, ESCH G, et al. Stimulated expression of TNF-a and IL-8, but not of lingual antimicrobial peptide reflects the concentration of pathogens contacting bovine mammary epithelial cells［J］. Vet Immunol Immunopathol, 2010, 135(1-2):152-157.

［17］陈仁金,杨章平,毛永江,等.中国荷斯坦牛IL8基因遗传多态性与泌乳性状以及体细胞评分的关联［J］.遗传, 2010, 32(12): 1256-1262.

［18］HEATON M P, CHITGO-MCGOWN C G, GROSSE W M, et al. Interleukin-8 haplotype structure from nucleotide sequence variation in commercial populations of US beef cattle［J］. Mamm Genome， 2001, 12(3): 219-226.

［19］LEYVA-BACA I, SCHENGEL F, SHARMA B S, et al. Identification of single nucleotide polymorphisms in the bovine CCL2, IL8, CCR2 and IL8RA genes and their association with health and production in Canadian Holsteins［J］. Anim Genet, 2007, 38(3): 198-202.

［20］刘坤,陈亮,毛永江,等.澳系进口荷斯坦牛初配月龄对其繁殖性能的影响［J］.中国奶牛,2011,24: 30-33.

［21］SAMBROOG J, FRITSCH E， MANIATIS T. Molecular Cloning: A Laboratory Manual［M］ , Cold Spring Harbor Laboratory, New York, 1989.

［22］王小龙，陈仁金，杨章平，等. 中国荷斯坦牛SCD1基因多态性与泌乳性状的关联分析［J］.畜牧兽医学报，2011，42(7):1022-1026.

［23］CHEN R J, YANG Z P, JI D J, et al. Polymorphisms of the IL8 gene correlate with milking traits,SCS and mRNA level in Chinese Holstein［J］. Mol Biol Report, 2011,38(6):4083-4088.

［24］WIGGANS G R, SHOOK G E. A lactation measure of somatic cell count［J］. J Dairy Sci, 1987, 70: 2666-2672.

［25］ROTTER V, NAGAEV I, SMITH U. Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is like IL-8 and tumor necrosis factor-a, over expressed in human fat cells from insulin-resistant subjects［J］. J Biol Chem，2003, 278(46): 45777-45784.

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澳系进口荷斯坦牛IL8基因遗传多态性与体细胞评分及泌乳性状的关联分析

Genetic Polymorphism of IL8 Gene and Its Association with Milk Traits and SCS in Holstein Imported from Australia

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